- 中国科技论文统计源期刊
- 中国科技核心期刊
| Citation: |
Hu Sheng-guo, Guo Yi, Yuan Chao, Li You-kong, Wang Min, Zhu Min. Role and mechanism of pumilio homolog 1 in hypoxia/reoxygenation induced injury of human renal tubular epithelial cells[J]. Journal of Clinical Nephrology, 2024, 24(10): 841-846. DOI: 10.3969/j.issn.1671-2390.2024.10.007
|
To explore the role and mechanism of pumilio homolog 1 （PUM1） in hypoxia/reoxygenation induced cell injury in human renal tubular epithelial cells （HK-2）.
A hypoxia/reoxygenation （H/R） model of HK-2 cells was established in vitro. PUM1 expression was knocked down through small interfering RNA （siRNA）. Cells were randomized into three groups of control, hypoxia/reoxygenation （H/R） and H/R+siRNA. Western blot （WB） method was used for detecting the expression level of PUM1 protein. Cell Count Kit 8 （CCK-8） was employed for detecting cell viability. Hydrogen peroxide （H2O2）, malondialdehyde （MDA） and superoxide dismutase （SOD） were used for evaluating the levels of oxidative stress. Flow cytometry was utilized for detecting the level of cell apoptosis.
As compared with control group, protein expression level of PUM1 in H/R 3 h group （1.76 ± 0.11 vs 0.98 ± 0.05）, H/R 6 h group （2.89 ± 0.14 vs 0.98 ± 0.05） and H/R 12 h group （3.78 ± 0.08 vs 0.98 ± 0.05） gradually spiked with the prolongation of hypoxic time. As compared with H/R group, knocking down the expression of PUM1 significantly improved the cell viability （73.67 ± 3.42 vs 29.60 ± 2.94）, oxidative stress [H2O2:（13.53 ± 0.85）μmol/L vs （22.43 ± 1.12）μmol/L, MDA: （16.03 ± 0.70）μmol/L vs （31.20 ± 1.50）μmol/L, SOD: （
PUM1 is up-regulated in H/R induced HK-2 cells and its inhibition may alleviate H/R injury through reducing oxidative stress and lowering cell apoptosis levels.
| [1] |
黄霞, 薛姗姗, 李苏萌, 等. USP14抑制对肾缺血再灌注诱导的急性肾损伤及IRE1/JNK信号通路的影响[J]. 临床肾脏病杂志,2023,23(8):663-669.DOI: 10.3969/j.issn.1671-2390.2023.08.008.
Huang X, Xue SS, Li SM, et al. Effects of USP14 inhibition on renal ischemia-reperfusion-induced acute kidney injury and IRE1/JNK signaling pathway[J]. J Clin Nephrol,2023,23(8):663-669. DOI: 10.3969/j.issn.1671-2390.2023.08.008.
|
| [2] |
Wang YP, Sun L, Wang LL, et al. PUM1 modulates trophoblast cell proliferation and migration through LRP6[J]. Biochem Cell Biol,2021,99(6):735-740. DOI: 10.1139/bcb-2021-0044.
|
| [3] |
Li X, Yang J, Chen X, et al. PUM1 represses CDKN1B translation and contributes to prostate cancer progression[J]. J Biomed Res,2021,35(5):371-382. DOI: 10.7555/JBR.35.20210067.
|
| [4] |
Yoon DS, Lee KM, Choi Y, et al. TLR4 downregulation by the RNA-binding protein PUM1 alleviates cellular aging and osteoarthritis[J]. Cell Death Differ,2022,29(7):1364-1378. DOI: 10.1038/s41418-021-00925-6.
|
| [5] |
Xia K, Qiu T, Jian YH, et al. Degradation of histone deacetylase 6 alleviates ROS-mediated apoptosis in renal ischemia-reperfusion injury[J]. Biomed Pharmacother,2023,165:115128. DOI:10.1016/ j.biopha.2023.115128.
|
| [6] |
柯心雨, 杨定平. 线粒体SIRT3在肾缺血再灌注损伤中作用的研究进展[J]. 临床肾脏病杂志,2021,21(5):421-426.DOI: 10.3969/j.issn.1671-2390.y20-143.
Ke XY, Yang DP. Research advances on role of mitochondrial SIRT3 during renal ischemia-reperfusion injury[J]. J Clin Nephrol,2021,21(5):421-426. DOI: 10.3969/j.issn.1671-2390.y20-143.
|
| [7] |
夏康, 王磊, 陈志远, 等. 组蛋白去乙酰化酶3在肾缺血再灌注诱导的急性肾损伤中的作用及其机制[J]. 中华实验外科杂志,2022,39(3):488-491.DOI: 10.3760/cma.j.cn421213-20210730-01229.
Xia K, Wang L, Chen ZY, et al. The role and mechanism of histone deacetylase 3 in acute kidney injury induced by renal ischemia-reperfusion[J]. Chin J Exp Surg,2022,39(3):488-491. DOI: 10.3760/cma.j.cn421213-20210730-01229.
|
| [8] |
Zhou JQ, Guo LN, Ma TF, et al. N-acetylgalactosaminyltransferase-4 protects against hepatic ischemia/reperfusion injury by blocking apoptosis signal-regulating kinase 1 N-terminal dimerization[J]. Hepatology,2022,75(6):1446-1460. DOI: 10.1002/hep.32202.
|
| [9] |
赵晓帅, 黄琴, 田浩, 等. REV-ERB激动剂SR9009通过上调SLC7A11/GPX4轴限制铁死亡减轻2型糖尿病大鼠心肌缺血再灌注损伤[J]. 医学研究杂志,2023,52(9):35-39.DOI: 10.11969/j.issn.1673-548X.2023.09.008.
Zhao XS, Huang Q, Tian H, et al. REV-ERB agonist SR9009 attenuates myocardial ischemia-reperfusion injury in type 2 diabetic rats by limiting iron death through up-regulation of SLC7A11/GPX4 axis[J]. J Med Res,2023,52(9):35-39. DOI: 10.11969/j.issn.1673-548X.2023.09.008.
|
| [10] |
Mao H, Zhang Y, Xiong YF, et al. Mitochondria-targeted antioxidant mitoquinone maintains mitochondrial homeostasis through the Sirt3-dependent pathway to mitigate oxidative damage caused by renal ischemia/reperfusion[J]. Oxid Med Cell Longev,2022,2022:2213503. DOI: 10.1155/2022/2213503.
|
| [11] |
Liu H, Wang W, Weng XD, et al. The H3K9 histone methyltransferase G9a modulates renal ischemia reperfusion injury by targeting Sirt1[J]. Free Radic Biol Med,2021,172:123-135. DOI: 10.1016/j.freeradbiomed.2021.06.002.
|
| [12] |
张晓林, 刘文丽, 李光远, 等. 基于NOD2信号通路探讨NADPH抑制剂对肾脏缺血再灌注损伤的影响[J]. 临床肾脏病杂志,2020,20(1):57-61.DOI: 10.3969/j.issn.1671-2390.2020.01.011.
Zhang XL, Liu WL, Li GY, et al. Investigation of effect of NADPH inhibitors on ischemic reperfusion injury based on the NOD2 signaling pathway[J]. J Clin Nephrol,2020,20(1):57-61. DOI: 10.3969/j.issn.1671-2390.2020.01.011.
|
| [13] |
梁海波, 邓晓凤, 臧敏, 等. Pumilio家族基因诱导性敲除小鼠模型的构建[J]. 南京医科大学学报(自然科学版),2023,43(8):1055-1060.DOI: 10.7655/NYDXBNS20230803.
Liang HB, Deng XF, Zang M, et al. An inducible knookout mouse model for Pumilio gene familiy[J]. J Nanjing Med Univ Nat Sci,2023,43(8):1055-1060. DOI: 10.7655/NYDXBNS20230803.
|
| [14] |
Gong YY, Liu ZK, Yuan YH, et al. PUMILIO proteins promote colorectal cancer growth via suppressing p21[J]. Nat Commun,2022,13(1):1627. DOI: 10.1038/s41467-022-29309-1.
|
| [15] |
Turner DJ, Turner M. RNA binding proteins As regulators of oxidative stress identified by a targeted CRISPR-Cas9 single guide RNA library[J]. CRISPR J,2021,4(3):427-437. DOI: 10.1089/crispr.2020.0116.
|
Supported by: Beijing Renhe Information Technology Co., Ltd. 
.png)
DownLoad: