The role and mechanism of long non-coding RNA ARID2-IR in chronic glomerulonephritis

Objective To investigate the relationship between LncRNA ARID2-IR and renal function in patients with chronic glomerulonephritis(CGN)and its mechanism.Methods A total of 52 CGN patients(CGN group)and 45 healthy adults(control group)were enrolled in the study.General information(age,gender,blood pressure,body mass index,etc.)was collected.Blood urea nitrogen(BUN)and serum creatinine(Scr)were tested.Target gene prediction was performed through the Starbase database,and KEGG pathway enrichment analysis was performed by Tbtools software.Real-time fluorescent quantitative PCR was used to detect the expression of LncRNA ARID2-IR and target genes.With ROC curve,the diagnostic value of LncRNA ARID2-IR and target genes for CGN was analyzed,and the influencing factors of CGN were analyzed by Logistic regression model.Results Systolic blood pressure,diastolic blood pressure,BUN and Scr in the CGN group were significantly higher than those in the control group(P<0.05).The expression of LncRNA ARID2-IR in the CGN group was significantly higher than that in the control group(P<0.05).LncRNA ARID2-IR expression was significantly correlated with BUN(r=0.225,P<0.05)and Scr(r=0.266,P<0.05),respectively.Target gene prediction showed that LncRNA ARID2-IR had 88 target genes in total,and the top 5 target genes in the score ranking for matching with LncRNA ARID2-IR were IFT80,IGHG4,RLBP1,ANKRD27 and TSPAN6,of which IFT80 and RLBP1 in the CGN group had much higher expression levels than those in the control group(P<0.05).The ROC curve showed that the AUCs of LncRNA ARID2-IR,IFT80 and RLBP1 were 0.783,0.821 and 0.738,respectively,which was of diagnostic significance for CGN.Logistic regression analysis showed that elevated levels of LncRNA ARID2-IR(OR=4.754,P=0.021),IFT80(OR=3.518,P=0.001)and RLBP1(OR=4.718,P=0.021)increased CGN risk.Conclusions Up-regulation of LncRNA ARID2-IR expression plays an important role in CGN.LncRNA ARID2-IR may be a new target for CGN control.