The study of antiproliferative and apoptosis-promoting effect of cinobufotalin on kidney cancer cells 786-O

Objective To study the antiproliferative and apoptosis-promoting effect of cinobufotalin on kidney cancer cells 786-O and its mechanism. Methods In vitro cultured kidney cancer cells 786-O were divided into control group,1 mg/mL cinobufotalin group, 10 mg/mL cinobufotalin group and 100 mg/mL cinobufotalin group.Cell proliferation inhibition rate was calculated, cell apoptosis rate wasmeasured by flow cytometry, nucleus apoptosis were detected by Hoechst-PI nuclear staining, and expressions of the protein Survivin were detected by western blot method. Results Cell proliferation inhibition rate increased with cinobufotalin concentrations, and exhibited time-dependent. Flow cytometry and Hoechst-PI nuclear staining test results showed that kidney cancer cells 786-O apoptosis rate increased in a dose-dependent manner as cinobufotalin concentration increased. Western blot showed that cinobufotalin inhibited the expression of survivin. Conclusions Cinobufotalin inhibits kidney cancer cells 786-O's proliferation and promotes cancer cell apoptosis by down-regulating the expression of Survivin.