Effects and mechanisms of telmisartan on adiponectin receptors in rat renal proximal tubular epithelial cells induced by high glucose

Objective To investigate the effects and mechanisms of telmisartan on expression of adiponectin receptors 1/2 in rat renal proximal tubular epithelial cells induced by high glucose.Methods Cultured rat renal proximal tubular epithelial cells (NRK-52E) which were synchronized in vitro were divided into 6 groups:normal control group (5.5 mmol/L glucose medium culture, 24h), hypertonic control group (5.5 mmol/L glucose medium with 19.5 mmol/L mannitol treatment, 24h), high glucose group (25 mmol/L glucose medium for 12h, 24h, 48h,72h respectively), high glucose+telmisartan group25 mmol/L glucose medium with different concentrations of telmisartan (1, 10, 100 nmol/L) for 24h, high glucose+telmisartan+GW9662 group (pretreatment with 5 000 nmol/L PPARγ blocker GW9662 for 30 min, then 25 mmol/L glucose and 100 nmol/L telmisartan for 24h), high glucose+GW9662 group (pretreatment with 5 000 nmol/L PPARγ blocker GW9662 for 30 min, then 25 mmol/L glucose for 24h). The reverse transcription time-polymerase chain reaction (RT-PCR) and Western blotting were used to detect the expression of adiponectin-receptors 1/2, PPAR-γ mRNA and protein levels in each group.Results In rat renal proximal tubular epithelial cells stimulated by high glucose, telmisartan could promote adiponectin receptors 1/2 expression through the pathway of PPAR-γ.Conclusions In rat renal proximal tubular epithelial cells stimulated by high glucose,telmisartan could promote adiponectin receptor 1/2 expression through the pathway of PPAR-γ.