SHI Chun-hua, MA Fei. Study on simvastatin improving the UUO rats renal fibrosis and anti-fibrosis plausible mechanism[J]. Journal of Clinical Nephrology, 2018, 18(11): 705-709. DOI: 10.3969/j.issn.1671-2390.2018.11.011
    Citation: SHI Chun-hua, MA Fei. Study on simvastatin improving the UUO rats renal fibrosis and anti-fibrosis plausible mechanism[J]. Journal of Clinical Nephrology, 2018, 18(11): 705-709. DOI: 10.3969/j.issn.1671-2390.2018.11.011

    Study on simvastatin improving the UUO rats renal fibrosis and anti-fibrosis plausible mechanism

    • Objective This study is aim to investigate simvastatin reducing the renal tissue fibrosis and the possible mechanism in unilateral ureteral obstruction (UUO) rats. Methods The 30 Sprague-Dawley (SD) rats were divided randomly into 3 groups:the sham group (Sham), the model group (UUO) and the simvastatin group (Sim). The simvastatin group had been treated with 3 mL the suspension of normal saline and Simrvastatin (20mg/kg/d) by daily gastric gavage, from three days before the UUO operation to the day of has been sacrificed, the Sham and model group were treated with the same dose normal saline in an identical fashion. each group were sacrificed respectively at 7 days after surgery. Using HE, PAS and Masson staining to observe the pathological changes in renal tissue. In addition, immunohistochemistry st-aining were employed to detect the expression of Wnt4 and β-catenin protein in renal tissue.And Western blotting were used to detect the expression of Wnt4, β-catenin, G-SK-3β、p-GSK-3β、E-cadherin, α-SMA and collagenⅠprotein in the rats renal tissue. Results Compared with the sham group, the typical morphologic changes were presenting in the model group, the expression of Wnt4,β-catenin,α-SMA,p-GSK-3β and collagenⅠprotein was increased significantly(P<0.05), while the levels of Ecadherin protein was obvious decreased (P<0.01).Compared with the model group, the renal fibrosis were obviously relief in the simvastatin group, accompanied by the levels of Wnt4, β-catenin,p-GSK-3β, α-SMA and collagenⅠprotein expression was obvious decreased(P<0.05), and the expression of Ecadherin protein was evident increased(P<0.05). Conclusions The simvastatin reduces the renal fibrosis in UUO rats, the possible mechanism, maybe relate to inhibiting the the activation and transduction of Wnt4/β-catenin signaling pathway.
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