Establishment of modified IgA nephropathy rat model and assessment of oxidative stress

GAO Ming; ZHANG Lian-dong; ZHANG Li; WANG Chang; LU Yan

doi:10.3969/j.issn.1671-2390.2018.04.011

Journal of Clinical Nephrology > 2018 > 18(4): 243-247. > DOI: 10.3969/j.issn.1671-2390.2018.04.011

GAO Ming, ZHANG Lian-dong, ZHANG Li, WANG Chang, LU Yan. Establishment of modified IgA nephropathy rat model and assessment of oxidative stress[J]. Journal of Clinical Nephrology, 2018, 18(4): 243-247. DOI: 10.3969/j.issn.1671-2390.2018.04.011

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Establishment of modified IgA nephropathy rat model and assessment of oxidative stress

Department of Nephrology, Xi'an No.4 Hospital, Xi'an 710003, China

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Received Date: June 26, 2017
Rev Recd Date: March 17, 2018
Available Online: May 11, 2023
Published Date: April 27, 2018

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Abstract

Objective To modify the traditional IgA nephropathy model and evaluate the oxidative stress status of the model, providing experimental evidence for the establishment of IgA nephropathy animal model.Methods Twelve female SPF SD rats weighing 200-240 g were randomly divided into normal control group (group C) and modified model group (group M) according to the random digital table method. The animals in group C were gavaged with normal saline at 4 ml/kg and given subcutaneous injection at 0.4 ml, and those in group M were treated with bovine serum albumin at 600 mg/kg every day by gavage, and subcutaneously injected with carbon tetrachloride at 0.10 ml, castor oil at 0.3 ml, thymosin at 3 mg and LPS at 0.05 mg. At the 12th week, the general states of rats in two groups were observed. Serum creatinine (SCr), blood urea nitrogen (BUN), albumin (Alb) and 24-h urine protein were determined. IgA immunofluorescence, HE staining, biochemical index as well as the state of oxidative stress in the kidney were evaluated.Results All the rats in the group M suffered from mental depression, tiredness, decreased movement and sparse hair. BUN and SCr levels in group M were significantly higher (P<0.05), the level of Alb was significantly lower (P<0.01), and 24-h urine protein was significantly higher than those in group C (P<0.05). In group M, IgA clumps were deposited in the renal mesangial region, the glomerular mesangial area was slightly widened, even moderately broadened, and increased mesangial cells and matrix were observed by HE staining. The levels of SOD and T-AOC in renal tissue of group M were significantly lower (P<0.05), and the level of MDA was significantly higher than that of group C (P<0.05).Conclusions The modified IgA nephropathy rat model is optimal, and the biochemical and pathological indexes are similar to those of human IgA nephropathy. The change of oxidative stress in the kidney is one of the important pathological mechanisms involved in the process.
- IgA nephropathy,
- Animal model,
- Oxidative stress

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[1]	Shu D, Xu F, Su Z, et al. Risk factors of progressive IgA nephropathy which progress to end stage renal disease within ten years:a case-control study[J]. BMC Nephrol, 2017, 18(1):11-16.
[2]	乐伟波, 梁少珊, 邓康平, 等. 1126例中国汉族成人IgA肾病患者的长期预后及危险因素分析[J]. 肾脏病与透析肾移植杂志, 2011, 20(2):101-108.
[3]	Kouchi M, Toyosawa K, Matsumoto I, et al. Hyaline glomerulopathy with tubulo-fibrillary deposits in young ddY mice[J]. Toxicol Pathol, 2011, 39(6):975-979.
[4]	李冬. 大鼠肝衰竭前期模型建立及辅助性T淋巴细胞17/调节性T淋巴细胞失衡的研究[J]. 临床肝胆病杂志, 2016, 32(5):928-932.
[5]	Li LS, Liu ZH. Epidemiologic data of renal diseases from a single unit in China:analysis based on 13,519 renal biopsies[J]. Kidney Int, 2004, 66(3):920-923.
[6]	李慧琼, 刘帝, 刘烨歆, 等. IgA肾病发病机制研究纵览[J]. 临床肾脏病杂志, 2016, 16(12):708-712.
[7]	Rifai A, Small PJ, Teague PO, et al. Experimental IgA nephropathy[J]. J Exp Med, 1979, 150(5):1161-1173.
[8]	Tomino Y. Spontaneous animal model, ddY mouse, for studying the pathogenesis and treatment in patients with immunoglobulin A nephropathy[J]. Nephrology(Carlton), 2010, 15(1):1-6.
[9]	Mestecky J, Novak J, Moldoveanu Z, et al. IgA nephropathy enigma[J]. Clin Immunol, 2016, 172:72-77.
[10]	Xin Q, Li J, Dang J, et al. miR-155 Deficiency ameliorates autoimmune inflammation of systemic lupus erythematosus by targeting S1pr1 in faslpr/lpr mice[J]. J Immunol, 2015, 194(11):5437-5445.
[11]	汤颖, 娄探奇, 成彩联, 等. 实验性IgA肾病模型的改进[J]. 中山大学学报(医学科学版), 2006, 27(2):184-187.
[12]	Sheng X, Zuo X, Liu X, et al. Crosstalk between TLR4 and Notch1 signaling in the IgA nephropathy during inflammatory response[J]. Int Urol Nephrol, 2017, 36(3):521-523.
[13]	Yeo SC, Cheung CK, Barratt J. New insights into the pathogenesis of IgA nephropathy[J]. Pediatr Nephrol, 2017, 42(3):463-470.
[14]	Soares MF, Roberts IS. IgA nephropathy:an update[J]. Curr Opin Nephrol Hypertens, 2017, 26(3):165-171.
[15]	Serafino A, Pierimarchi P, Pica F, et al. Thymosin alpha1 as a stimulatory agent of innate cell-mediated immune response[J]. Ann N Y Acad Sci, 2012, 1270:13-20.
[16]	Liu F, Wang HM, Wang T, et al. The efficacy of thymosin alpha1 as immunomodulatory treatment for sepsis:a systematic review of randomized controlled trials[J]. BMC Infect Dis, 2016, 16(3):488-492
[17]	李冬, 陆忠华, 甘建和. 肝衰竭前期大鼠模型的建立及辅助性T淋巴细胞17/调节性T淋巴细胞失衡的研究[J]. 临床肝胆病杂志, 2016, 32(5):928-932.
[18]	Han C, Zhang L, Zhu X, et al. Plasma gelsolin levels are decreased and correlate with fibrosis in IgA nephropathy[J]. Exp Biol Med (Maywood), 2013, 238(11):1318-1327.
[19]	Pei Y, Xu Y, Ruan J, et al. Plasma oxidative stress level of IgA nephropathy in children and the effect of early intervention with angiotensin-converting enzyme inhibitors[J]. J Renin Angiotensin Aldosterone Syst, 2016, 17(2):160-165.
[20]	Zhu C, Mertens PR. IgA nephropathy and oxidative stress:news on clinically evaluated biomarkers hits the stage[J]. Int Urol Nephrol, 2012, 44(4):1277-1280.
[21]	Tanaka S, Ninomiya T, Masutani K, et al. Prognostic impact of serum bilirubin level on long-term renal survival in IgA nephropathy[J]. Clin Exp Nephrol, 2015, 19(6):1062-1070.
[22]	Wang Y, Tian J, Guo H, et al. Intermedin ameliorates IgA nephropathy by inhibition of oxidative stress and inflammation[J]. Clin Exp Med, 2016, 16(2):183-192.

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