The effects of canonical Wnt signaling pathways on the CXCR4 expression of bone marrow derived mesenchymal stem cells

Objective To evaluate the effects of Wnt signaling pathways on the CXCR4 expression of bone marrow mesenchymal stem cells(BMSCs), and to investigate the possible regulation mechanisms of the Wnt signaling pathways on the migration ability of BMSCs.Methods BMSCs were obtained from the femurs, tibias, humeri and radius of adult male SD rats. The expression of Wnt signaling pathway downstream target gene β-catenin was detected via RT-PCR and western-blotting after activating and blocking Wnt signaling pathway of BMSs. Meanwhile, expression of CXCR4 was also detected via RT-PCR and western-blotting after activating and blocking Wnt signaling pathway of BMSCs.Results (1)Dentification of BMSCs:BMSCs could expression CD29 and CD90, which were both marker of mesenchymal stem cells. Besides, BMSCs could express nerve culluar marker such as NSE, GFAP, and could be induced to differentiate into adipocytic cells, suggesting multi-directional differentiation.(2)Wnt3a (200 ng/ml) could fully activate Wnt signaling pathway in 24 h, with high expression level of β-catenin(compared with control group, P<0.05). While DKK-1 (100 ng/ml) could fully blockade Wnt signaling pathway in 24 h (compared with control group, P<0.05). (3)When the Wnt signaling pathway was blockaded, expression levels of CXCR4 in BMSCs was obviously increased (compared with control group, P<0.05); while the activated of such signaling pathway could decrease the expression of CXCR4 of BMSCs (compared with control group, P<0.05).Conclusions Wnt signaling pathway could regulate the expression of CXCR4 of BMSCs, and such regulation may promote the migration of BMSCs to kidney.