• Journals Master List
  • DOAJ
  • 数据库logo
  • EuroPub
ZHANG Jiong, WANG Jia, WANG Fang, LI Gui-sen. Protective effect of erythropoietin preconditioning on kidney ischemia-reperfusion injury[J]. Journal of Clinical Nephrology, 2017, 17(9): 564-568. DOI: 10.3969/j.issn.1671-2390.2017.09.012
Citation: ZHANG Jiong, WANG Jia, WANG Fang, LI Gui-sen. Protective effect of erythropoietin preconditioning on kidney ischemia-reperfusion injury[J]. Journal of Clinical Nephrology, 2017, 17(9): 564-568. DOI: 10.3969/j.issn.1671-2390.2017.09.012

Protective effect of erythropoietin preconditioning on kidney ischemia-reperfusion injury

More Information
  • Received Date: November 21, 2016
  • Rev Recd Date: August 14, 2017
  • Available Online: May 11, 2023
  • Published Date: September 27, 2017
  • Objective To explore the protective effect of erythropoietin (EPO) on renal ischemia-reperfusion injury (IRI) in rats.Methods Thirty male SD rats were randomly divided into sham operation group (Sham), renal IRI group (IRI) and EPO pretreatment group (EPO), 10 rats in each group. The renal IRI model was constructed by clamping the left renal pedicle for 1 h, removing the right kidney, and reperfusion for 24 h. In EPO group, EPO (1 000 U/kg) was intraperitoneally injected at 1 h before operation, while Sham group and IRI group were intraperitoneally injected with equal volume of normal saline. The left renal pedicle was clamped for 45 min in EPO and IRI groups, but not in Sham group. Renal perfusion was restored for 24 h, and then kidneys and serum were obtained. Enzyme linked immunosorbent assay (ELISA) was used to detect serum creatinine (SCr), blood urea nitrogen (BUN), pro-inflammatory cytokines[interleukin-6 (IL-6); interferon gamma (IFN-γ) and tumor necrosis factor alpha (TNF-α)] and anti-inflammatory cytokine (IL-10 and TGF-β). The reverse transcriptase polymerase chain reaction (RT-PCR) was used to detect renal pro-inflammatory cytokines (IL-6, IFN- γ and TNF-α) and anti-inflammatory cytokines[IL-10 and transforming growth factor beta (TGF-β)] mRNA expression levels. Western blotting was used to detect EPO receptor (EPOR), phosphorylated EPOR (p-EPOR), tyrosine kinase-2 (JAK2), signal transducer and activator of transcription 3 (STAT3), phosphorylated JAK2 (p-JAK2), phosphorylated STAT3 (p-STAT3) protein expression levels. Periodic acid Schiff staining (PAS) was used to examine the pathological changes of renal tissues.Results As compared with Sham group, the expression levels of p-EPOR, p-JAK2, p-STAT3, SCr, BUN, IL-6, TNF-α and IFN-γ were significantly increased, pathological changes of renal tissues obviously alleviated, and expression levels of IL-10 and TGF-β were markedly reduced in IRI group. There was no significant difference in EPOR, JAK2 and STAT3 levels between Sham group and IRI group. As compared with IRI group, the expression levels of p-EPOR, p-JAK2, p-STAT3, IL-10 and TGF-β were significantly increased, the levels of SCr, BUN, IL-6, TNF-α and IFN-γ were significantly reduced, and the pathological changes of renal tissues were significantly alleviated in EPO group, but there was no significant difference in EPOR, JAK2 and STAT3 expression between IRI group and EPO group.Conclusions EPO preconditioning can alleviate inflammation by inhibiting renal IRI, which may be related with the activation of EPOR/JAK2/STAT3 signaling pathway.
  • [1]
    Kusch A, Hoff U, Bubalo G, et al. Novel signalling mechanisms and targets in renal ischaemia and reperfusion injury[J]. Acta Physiol(Oxf), 2013, 208(1):25-40.
    [2]
    Yang Y, Song M, Liu Y, et al. Renoprotective approaches and strategies in acute kidney injury[J]. Pharmacol Ther, 2016, 163:58-73.
    [3]
    de Haan JE, Hoorn EJ, de Geus HRH. Acute kidney injury after liver transplantation:Recent insights and future perspectives[J]. Best Pract Res Clin Gastroenterol, 2017, 31(2):161-169.
    [4]
    Francis A, Baynosa R. Ischaemia-reperfusion injury and hyperbaric oxygen pathways:a review of cellular mechanisms[J]. Diving Hyperb Med, 2017, 47(2):110-117.
    [5]
    Yang J, Xiao Z, Li T, et al. Erythropoietin promotes the growth of pituitary adenomas by enhancing angiogenesis[J]. Int J Oncol, 2012, 40(4):1230-1237.
    [6]
    Bartnicki P, Kowalczyk M, Rysz J. The influence of the pleiotropic action of erythropoietin and its derivatives on nephroprotection[J]. Med Sci Monit, 2013, 19(1):599-605.
    [7]
    Nairz M, Sonnweber T, Schroll A, et al. The pleiotropic effects of erythropoietin in infection and inflammation[J]. Microbes Infect, 2012, 14(3):238-246.
    [8]
    Zhang J, Zou YR, Zhong X, et al. Erythropoietin pretreatment ameliorates renal ischaemia-reperfusion injury by activating PI3K/Akt signalling[J]. Nephrology (Carlton), 2015, 20(4):266-272.
    [9]
    Ardalan MR, Estakhri R, Hajipour B, et al. Erythropoietin ameliorates oxidative stress and tissue injury following renal ischemia/reperfusion in rat kidney and lung[J]. Med Princ Pract. 2013, 22(1):70-74.
    [10]
    Li Y, Zhu W, Tao J, et al. Fasudil protects the heart against ischemia-reperfusion injury by attenuating endoplasmic reticulum stress and modulating SERCA activity:the differential role for PI3K/Akt and JAK2/STAT3 signaling pathways[J]. PloS One, 2012, 7(10):e48115.
    [11]
    Bamgbola OF. Spectrum of anemia after kidney transplantation:pathophysiology and therapeutic implications[J]. Clin Transplant, 2016, 30(10):1185-1194.
    [12]
    Doi K, Rabb H. Impact of acute kidney injury on distant organ function:recent findings and potential therapeutic targets[J]. Kidney Int, 2016, 89(3):555-564.
    [13]
    Palagani V, Bozko P, El Khatib M, et al. Combined inhibition of Notch and JAK/STAT is superior to monotherapies and impairs pancreatic cancer progression[J]. Carcinogenesis, 2014, 35(4):859-866.
    [14]
    Yu HC, Qin HY, He F, et al. Canonical notch pathway protects hepatocytes from ischemia/reperfusion injury in mice by repressing reactive oxygen species production through JAK2/STAT3 signaling[J]. Hepatology, 2011, 54(3):979-988.
    [15]
    Zhu H, Zou L, Tian J, et al. SMND-309, a novel derivative of salvianolic acid B, protects rat brains ischemia and reperfusion injury by targeting the JAK2/STAT3 pathway[J]. Eur J Pharmacol, 2013, 714(1-3):23-31.
  • Related Articles

    [1]Niu Yun-he, Bao Ying, Huang Hui-mei, Wang Yan-ping, Li Zhi-juan, Zhang Min, Wang Ying, Liang Nan. Hemolytic-uremic syndrome plus IgA nephropathy in children: one case report with a literature review[J]. Journal of Clinical Nephrology, 2024, 24(12): 1049-1052. DOI: 10.3969/j.issn.1671-2390.2024.12.012
    [2]Lin Yan-duan, Li rui, Fu Qiao-min, Chen Jiang-hua, Li Kun-jian, Zhou Xiao-feng, Chen Hui-pin. Effect of percutaneous injection of hemostatic agents guided by ultrasound for arresting active hemorrhage after renal biopsy[J]. Journal of Clinical Nephrology, 2024, 24(5): 364-369. DOI: 10.3969/j.issn.1671-2390.2024.05.003
    [3]Zhu Li-na, Zhang Jiong. Recurrent gross hematuria and acute kidney injury after heart valve replacement: one report[J]. Journal of Clinical Nephrology, 2024, 24(3): 257-260. DOI: 10.3969/j.issn.1671-2390.2024.03.013
    [4]Zhang Hong-wen. Diagnostic procedure of hematuria in children[J]. Journal of Clinical Nephrology, 2023, 23(5): 353-356. DOI: 10.3969/j.issn.1671-2390.2023.05.001
    [5]Long Meng-tuan, Zhu Xiao-yu, Wei Xue-jiao, Zhao Dan, Jiang Li-li, Du Yu-jun. Role of magnesium in chronic kidney disease complications[J]. Journal of Clinical Nephrology, 2021, 21(12): 1033-1040. DOI: 10.3969/j.issn.1671-2390.2021.12.012
    [6]Xiao Shu-na, Xia Zhi, Cheng Ying, Li Yong, Shi Bu-yun, Tang Wen, Zhu Song-bai, Qing Cheng-guang, Xu Hui, Huang Cheng-jiao. Hemolytic uremic syndrome in children: a report of four cases with a literature review[J]. Journal of Clinical Nephrology, 2021, 21(3): 262-265. DOI: 10.3969/j.issn.1671-2390.w20-155
    [7]LI Chun-zhen, SUN Chao, ZHANG Dong-feng, LIU Ling, LIU Fu-juan, GE Lan-lan, YUAN Xiao-ying, YIN Mei-na, YANG Yan-jun, CUI Jie-yuan. Clinical and pathological features of Henoch-Schonlein purpura nephritis with macrohematuria at the early disease stage[J]. Journal of Clinical Nephrology, 2020, 20(9): 736-739. DOI: 10.3969/j.issn.1671-2390.2020.09.009
    [8]DUAN Rui-qiu, ZHAO Wei, ZHAO Hai-dan. The research of the relationship between serum uric acid level and all-cause mortality at the beginning of maintenance hemodialysis and one year after diagnosis in hemodialysis patients[J]. Journal of Clinical Nephrology, 2020, 20(8): 653-656. DOI: 10.3969/j.issn.1671-2390.2020.08.008
    [9]SHEN Jing-jing, YANG Wen-jing, WANG Ying. Clinical observation of continuous blood purification for treatment of pediatric hemolytic uremic syndrome[J]. Journal of Clinical Nephrology, 2019, 19(9): 697-699. DOI: 10.3969/j.issn.1671-2390.2019.09.012
    [10]QIU Cai-xia, XU Yan-yan, RAO Xiao-pang. Relationship between serum dehydroepiandrosterone and the level of blood uric acid in non-obese males[J]. Journal of Clinical Nephrology, 2017, 17(6): 352-355. DOI: 10.3969/j.issn.1671-2390.2017.06.007

Catalog

    Article views (12) PDF downloads (316) Cited by()

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return