张爱萍, 贺芳, 佘开儒. 长非编码RNA ARID2-IR在慢性肾小球肾炎中的作用及机制研究[J]. 临床肾脏病杂志, 2020, 20(10): 786-791. DOI: 10.3969/j.issn.1671-2390.2020.10.004
    引用本文: 张爱萍, 贺芳, 佘开儒. 长非编码RNA ARID2-IR在慢性肾小球肾炎中的作用及机制研究[J]. 临床肾脏病杂志, 2020, 20(10): 786-791. DOI: 10.3969/j.issn.1671-2390.2020.10.004
    shu
    ZHANG Ai-ping, HE Fang, SHE Kai-ru. The role and mechanism of long non-coding RNA ARID2-IR in chronic glomerulonephritis[J]. Journal of Clinical Nephrology, 2020, 20(10): 786-791. DOI: 10.3969/j.issn.1671-2390.2020.10.004
    Citation: ZHANG Ai-ping, HE Fang, SHE Kai-ru. The role and mechanism of long non-coding RNA ARID2-IR in chronic glomerulonephritis[J]. Journal of Clinical Nephrology, 2020, 20(10): 786-791. DOI: 10.3969/j.issn.1671-2390.2020.10.004
    shu

    长非编码RNA ARID2-IR在慢性肾小球肾炎中的作用及机制研究

    The role and mechanism of long non-coding RNA ARID2-IR in chronic glomerulonephritis

      摘要
    • 摘要: 目的 探讨慢性肾小球肾炎（chronic glomerulonephritis，CGN）人群中长非编码RNA（long non-coding RNA，LncRNA）ARID2-IR与肾功能的相关性及其机制。方法 共有52例CGN患者（CGN组）和45名健康成年人（对照组）纳入本研究。收集两组一般资料（年龄、性别、血压、体质量指数等），检测血尿素氮、血肌酐水平。通过Starbase数据库进行靶基因预测，通过Tbtools软件进行KEGG通路富集分析。采用实时荧光定量PCR检测LncRNA ARID2-IR和靶基因的表达。采用ROC曲线分析LncRNA ARID2-IR和靶基因对于CGN的诊断价值。采用Logistic回归分析CGN的影响因素。结果 CGN组的收缩压、舒张压、血尿素氮和血肌酐均显著高于对照组（P<0.05）。CGN组LncRNA ARID2-IR的表达显著高于对照组（P<0.05）。LncRNA ARID2-IR表达分别与血尿素氮（r=0.225，P<0.05）和血肌酐（r=0.266，P<0.05）呈显著正相关。靶基因预测显示LncRNA ARID2-IR共有88个靶基因，与LncRNA ARID2-IR匹配得分排名前5的靶基因为IFT80、IGHG4、RLBP1、ANKRD27和TSPAN6，其中IFT80和RLBP1在CGN组中的表达水平显著高于对照组（P<0.05）。ROC曲线显示，LncRNA ARID2-IR、IFT80和RLBP1预测CGN的曲线下面积分别为0.783、0.821和0.738，对于CGN具有诊断意义。Logistic回归分析显示，LncRNA ARID2-IR（OR=4.754，P=0.021）、IFT80（OR=3.518，P=0.001）和RLBP1（OR=4.718，P=0.021）水平升高会增加患CGN的风险。结论 LncRNA ARID2-IR的表达上调在CGN中发挥了重要作用，LncRNA ARID2-IR可能是CGN防治的新靶点。

       

      Abstract: Objective To investigate the relationship between LncRNA ARID2-IR and renal function in patients with chronic glomerulonephritis(CGN)and its mechanism.Methods A total of 52 CGN patients(CGN group)and 45 healthy adults(control group)were enrolled in the study.General information(age,gender,blood pressure,body mass index,etc.)was collected.Blood urea nitrogen(BUN)and serum creatinine(Scr)were tested.Target gene prediction was performed through the Starbase database,and KEGG pathway enrichment analysis was performed by Tbtools software.Real-time fluorescent quantitative PCR was used to detect the expression of LncRNA ARID2-IR and target genes.With ROC curve,the diagnostic value of LncRNA ARID2-IR and target genes for CGN was analyzed,and the influencing factors of CGN were analyzed by Logistic regression model.Results Systolic blood pressure,diastolic blood pressure,BUN and Scr in the CGN group were significantly higher than those in the control group(P<0.05).The expression of LncRNA ARID2-IR in the CGN group was significantly higher than that in the control group(P<0.05).LncRNA ARID2-IR expression was significantly correlated with BUN(r=0.225,P<0.05)and Scr(r=0.266,P<0.05),respectively.Target gene prediction showed that LncRNA ARID2-IR had 88 target genes in total,and the top 5 target genes in the score ranking for matching with LncRNA ARID2-IR were IFT80,IGHG4,RLBP1,ANKRD27 and TSPAN6,of which IFT80 and RLBP1 in the CGN group had much higher expression levels than those in the control group(P<0.05).The ROC curve showed that the AUCs of LncRNA ARID2-IR,IFT80 and RLBP1 were 0.783,0.821 and 0.738,respectively,which was of diagnostic significance for CGN.Logistic regression analysis showed that elevated levels of LncRNA ARID2-IR(OR=4.754,P=0.021),IFT80(OR=3.518,P=0.001)and RLBP1(OR=4.718,P=0.021)increased CGN risk.Conclusions Up-regulation of LncRNA ARID2-IR expression plays an important role in CGN.LncRNA ARID2-IR may be a new target for CGN control.

       

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