Abstract: Objective To explore the significance of early detection of renal tubular markers in the high risk population with chronic kidney diseases (CKD), and analyze the influencing factors of abnormal increase of renal tubular injury markers. Methods According to the inclusion and exclusion criteria, 266 subjects were included in the study. The general clinical data, biochemical blood parameters, urine microalbumin-creatinine ratio(ACR), renal tubular injury indicesincluding Beta2-Microglobulin(β₂-MG), Alpha1-microglobulin(α₁-MG), Retinol Binding Protein (RBP) were recorded. The subjects were divided into positive and negative groups based on urinary ACR. The significance of early detection of renal tubular injury markers and the related influencing factors were analyzed. Results The positive rate of renal tubular injury in the urine ACR positive group was 86.36%, higher than that in the urine ACR negative group. However, in the urine ACR negative, 48.72% of patients had abnormal increase of renal tubular injury markers. Abnormal amplitude and abnormal item number of renal tubular injury markers in the urine ACR positive group were significantly higher than those in the negative group, with statistically significant difference (P<0.05). The ages of patients in the positive group were significantly higher than that in the negative group, with statistically significant difference (P<0.05). Multivariate stepwise regression analysis suggested that blood uric acid (OR=0.992, P=0.009) was a correlative factor for abnormal increase of renal tubular injury markers. Conclusions The detection of renal tubular protein has high clinical application value for the detection of early renal injury.